physarum polycephalum temperature
1980;179(1):43-8. doi: 10.1007/BF00268444. 1976 Nov 24;149(1):115-9. doi: 10.1007/BF00275966. We believe that this mutant, MA67, is affected in a step in the nuclear replication cycle occurring late in G2. Access everything in the JPASS collection, Download up to 10 article PDFs to save and keep, Download up to 120 article PDFs to save and keep. J Comput Theore Nanosci 8(3): 383-390. Under the conditions of the above experiments, sclerotia cannot be produced at high temperatures (32.5⚬-35.0⚬) nor at low temperatures (8⚬-12⚬). Temperature, humidit... Time lapse video showing about two and a half days growth of a plasmodium of physarum polycephalum, with one frame for every 30 seconds. Full Title: Sensory fusion in Physarum polycephalum and implementing multi-sensory functional computation Authors: James G.H. Speed maximum and half period length of the shuttle stream of Physarum polycephalum on the clinostat; temperature 20.5 (±0.3)° C. Tso WW, Mansour TE (1975) Thermotaxis in a slime mold, Physarum Molecular and General Genetics MGG, Vol. Laffler TG, Wilkins A, Selvig S, Warren N, Kleinschmidt A, Dove WF. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. Curr Genet. Physarum polycephalum belongs to the Amoebozoa, the sister group to the Opisthokonts (i.e., fungi and animals) ( Cavalier-Smith 2003) which both together form the supergroup Amorphea ( … This item is part of a JSTOR Collection. option. | Regulation of protein synthesis in the plasmodial phase of Physarum polycephalum. Photochem. 1980 Aug;143(2):897-905. doi: 10.1128/JB.143.2.897-905.1980. Access supplemental materials and multimedia. American Journal of Botany Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. This site needs JavaScript to work properly. Eur J Biochem. 64, … The interrelationships of the factors of temperature and pH upon the fruiting of Physarum polycephalum Schw. Michel Wright, Yvette Tollon, Regulation of thymidine kinase synthesis during the cell cycle of Physarum polycephalum by the heat-sensitive system which triggers mitosis and S phase, Experimental Cell Research, 10.1016/0014-4827(79)90304-5, 122, 2, (273-279), (1979). | Isolation of cell cycle mutants of Physarum polycephalum. This stage of the life cycle, along with its preference for damp shady habitats, likely contributed to the original mischaracterization of the organism as a fungus. For terms and use, please refer to our Terms and Conditions Theory of time-resolved somatic complementation and its use to explore the sporulation control network in Physarum polycephalum. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. The higher the temperature at which a plasmodium is maintained, the greater the acidity must be in order to produce fruiting. With pH remaining constant, (a) the time required for fruiting varies directly with temperature -i.e., the higher the temperature the longer the time required for fruiting; (b) the percentage of cultures producing fruiting bodies varies inversely with temperature-i.e., the higher the temperature, the smaller the percentage fruiting. On the day before the measurement, dried P. polycephalumwas placed on an agarose gel and lightly sprayed with water to induce germination. 1976 Aug 2;146(3):313-5 -. Physarum polycephalum Temperature compensation Access options Buy single article Instant access to the full article PDF. 1977 Aug;131(2):473-6 ©2000-2021 ITHAKA. In the Kateb and Costello study , a total of 87 compounds and 79 compounds were identified at the incubation temperature of 75 C and 30 C, respectively, from the plasmodia of P. polycephalum. Lane 1 , 0 min after temperature shift; Lane 2 , 1 min after temperature shift; Lane 3 , 2 min after temperature shift; Lane 4 , 5 min after temperature shift; Lane 5 , 10 min after temperature shift. Temperature-sensitive mutants of Physarum polycephalum: viability, growth, and nuclear replication. Physarum polycephalum (Slime mold) Status Reviewed-Annotation score: -Experimental evidence at protein level i Function i Catalytic activity i Milk clotting activity. The negative selectant, netropsin, was used to enrich for temperature-sensitive mutants among a population of mutagenized amoebae, and, separately, a nonselective screening method was used to isolate plasmodial temperature-sensitive mutants among clonal plasmodia derived from mutagenized amoebae. USA.gov. Physarum polycephalum, an acellular slime mold or myxomycete, is a protist with diverse cellular forms and broad geographic distribution. Wiley has partnerships with many of the world’s leading societies and publishes over 1,500 peer-reviewed journals and 1,500+ new books annually in print and online, as well as databases, major reference works and laboratory protocols in STMS subjects. The plasmodium of Physarum polycephalum is a unicellular and multinuclear giant amoeba that shows adaptive behaviors. AB259870). • Physarum is subcultured onto Physarum agar, and is incubated at room temperature or 25 C. To maintain viability, plasmodial To maintain viability, plasmodial Physarum should be subcultured weekly. Please enable it to take advantage of the complete set of features! Physarum polycephalum was cloned from a cDNA library, and the nucleotide sequence of the cDNA was determined (accession no. 3) Physarum polycephalum shows negative phototaxis against UV-blue light. Wiley is a global provider of content and content-enabled workflow solutions in areas of scientific, technical, medical, and scholarly research; professional development; and education. 1978 Mar;43(3):449-59 Generally speaking, the necessity of light for fruiting cannot be dispensed with by increasing acidity nor by lowering the temperature. 163, Issue. de Lacy Costello 1,2, Andrew Adamatzky 1.1 Unconventional Computing Centre, University of the West of England, Bristol, UK. The slime mould, Physarum polycephalum, meets a Danish cousin, Badhamia - and gets seriously bullied. In the setup illustrated in Fig. This same relationship exists for plasmodia maintained at low temperatures (8⚬-12⚬C.). COVID-19 is an emerging, rapidly evolving situation. Variations in the surrounding temperature altered membrane activities at the critical temperature, T c =15 C in plasmodium of the true slime mold Physarum polycephalum. Select the purchase NIH 1977 Dec;12(4):1109-20 The latter shows a striking reduction in the frequency of postmitotic reconstruction nuclei at the restrictive temperature. 1980 Apr;1(3):203-8. doi: 10.1007/BF00390945. Temperature-sensitive mutants of the slime mould Physarum polycephalum. The thermotaxis of the plasmodium is so strong and the organism without learning always proceeds to the higher temperature region. Mol Gen Genet. 163, Issue. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways. © 1939 Wiley Physarum Polycephalum changes polyaniline properties Alice Dimonte 1, Tatiana Berzina 1 and Victor Erokhin 1 1 CNR-IMEM (National Council of the Researches ± Institute of Materials for Electronics and Magnetism), Parco Area delle Of the two lytic mutants, one affected both amoebae and plasmodia, and the other affected plasmodia alone. In the absence of oxygen, no negative J Bacteriol. 1, p. 91. 1981 Dec;120(3):529-34. doi: 10.1111/j.1432-1033.1981.tb05732.x. A gene conferring actidione resistance and abnormal morphology on Physarum polycephalum plasmodia - Volume 15 Issue 1 - Jennifer Dee, Russell T. M. Poulter Skip to main content Accessibility help We use cookies to distinguish you from other users … The “acellular” moniker derives from the plasmodial stage of the life cycle: the plasmodium is a bright yellow macroscopic multinucleate coenocyte shaped in a network of interlaced tubes. Molecular and General Genetics MGG, Vol. -, Genet Res. have been studied, and from data derived from observations made on 2,030 cultures, the following conclusions are drawn: The factors of pH and temperature are closely related and interdependent. J Bacteriol. -, Mol Gen Genet. 1975 Jun;62(6):283-9. doi: 10.1007/BF00608954. Phototaxis in Physarum polycephalum 271 A tungsten 8V-30W microscope lamp (Olympus Co., Tokyo) was used to observe proto-plasmic … JSTOR®, the JSTOR logo, JPASS®, Artstor®, Reveal Digital™ and ITHAKA® are registered trademarks of ITHAKA. Heat sensitive factor necessary for mitosis onset in Physarum polycephalum (temperature shift/heat shock/cycloheximide/ts mutant). II. Wiley has published the works of more than 450 Nobel laureates in all categories: Literature, Economics, Physiology or Medicine, Physics, Chemistry, and Peace. Read your article online and download the PDF from your email or your account. Photobiol. It was then put in a darkroom with a constant temperature of 20°C. Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. 1, p. 1975 Dec;26(3):237-54 movement of Physarum polycephalum M Iima et al Studies of the phase gradient at the boundary of the phase diffusion equation, motivated by peculiar wave patterns of rhythmic contraction in the amoeboid movement of Printed in -, Toxicol Appl Pharmacol. Action spectrum was determined, which accords well with action spectrum for generation of super oxide anion. With temperature a constant factor, (a) highest percentage fruiting is obtained at pH 3.0, gradually decreasing as the pH value is increased; (b) least time is required for fruiting at pH 4.0. @article{osti_22391127, title = {Reversible logic gates on Physarum Polycephalum}, author = {Schumann, Andrew}, abstractNote = {In this paper, we consider possibilities how to implement asynchronous sequential logic gates and quantum-style reversible logic gates on Physarum polycephalum motions. All Rights Reserved. When transferred to the restrictive temperature, two mutants immediately lysed, whereas the remainder slowed or stopped growing. The growth-defective mutants were examined for protein and deoxyribonucleic acid synthesis and for aberrations in mitotic behavior. -, Cell. Cycloheximide resistance of Physarum polycephalum. Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature. Request Permissions. Tanaka Y, Nakagaki T (2011) Cellular computation realizing intelligence of slime mold Physarum Polycephalum. [Modern research on the cell cycle and mitosis in the myxomycete Physarum polycephalum]. HHS The transition temperature was constant at 16 C when plasmodia had been cultured above 16 C, and decreased gradually as the culture temperature decreased below … Changes in the membrane and zeta potentials, which were induced by chemical stimuli, decreased when the temperature decreased below T c . Execution of this step is necessary for both mitosis and chromosome replication. Naturwissenschaften. JSTOR is part of ITHAKA, a not-for-profit organization helping the academic community use digital technologies to preserve the scholarly record and to advance research and teaching in sustainable ways. AJB publishes peer-reviewed, innovative, significant research of interest to a wide audience of scientists in all areas of plant biology (e.g., biodiversity, structure, function, development, genetics, evolution, reproduction, systematics), all levels of organization (molecular to ecosystem), and all plant groups and allied organisms (cyanobacteria, algae, fungi, and lichens). Action spectrum for sporulation and photoavoidance in the plasmodium of Physarum polycephalum, as modified differentially by temperature and starvation. Check out using a credit card or bank account with. Thin layer chromatogram of crude lipids from Physarum cells at various periods after temperature shift from 24 C to 40 C. Lipids in each lane were extracted from 6.4 × 10 6 cells. The petri dishes were wrapped in aluminum foil at room temperature (22-25°C) and sealed with parafilm. As stated before, pH values at this temperature Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised Mol Gen Genet. Complementation in heterokaryons was used to sort the mutants into nine functional groups. Our online platform, Wiley Online Library (wileyonlinelibrary.com) is one of the world’s most extensive multidisciplinary collections of online resources, covering life, health, social and physical sciences, and humanities. Length of survival of plasmodia at high temperatures is dependent upon pH value and varies inversely with it. Mutants of the plasmodial phase. With a growing open access offering, Wiley is committed to the widest possible dissemination of and access to the content we publish and supports all sustainable models of access. Would you like email updates of new search results? P. polycephalum is used as a model organ… Maximum temperature at which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C. NLM Whiting 1, Ben P.J. 3, we prepared two thermal conditions in one agar plate using Peltier devices. Using a selfing strain of Physarum polycephalum that forms haploid plasmodia, we have isolated temperature-sensitive growth mutants in … The physarum polycephalum in form of sklerotia was cultivated in petri dishes covered with 1,5 and 2% agar-agar and pour with some drops of water. The higher the pH value, the shorter the period of time a plasmodium will live. One mutant may be defective in both protein and deoxyribonucleic acid synthesis, and another only in deoxyribonucleic acid synthesis. Clipboard, Search History, and several other advanced features are temporarily unavailable. The P. polycephalumcultured on the gel vividly about 12 hours later. Our core businesses produce scientific, technical, medical, and scholarly journals, reference works, books, database services, and advertising; professional books, subscription products, certification and training services and online applications; and education content and services including integrated online teaching and learning resources for undergraduate and graduate students and lifelong learners. Founded in 1807, John Wiley & Sons, Inc. has been a valued source of information and understanding for more than 200 years, helping people around the world meet their needs and fulfill their aspirations. Action Spectrum for Sporulation and Photoavoidance in the Plasmodium of Physarum polycephalum, as Modified Differentially by Temperature and Starvation Toshiyuki Nakagaki Corresponding Author Published by the Botanical Society of America continuously since 1914, the American Journal of Botany (AJB) is the Society’s flagship research journal. Sterilized whole meal oat flakes were served as food and positioned near of the slime mold. Since Howard stated that the optimum temperature for P. polycephalum is between 20.00 and 26.0?C., the first of the present series was conducted within this temperature range. In the temperature range around 10–30 °C, the plasmodium shows thermotaxis to the higher temperature. | Ph value and varies inversely with it striking reduction in the myxomycete Physarum polycephalum temperature Access... Is so strong and the organism without learning always proceeds to the higher the temperature ):529-34.:. Myxomycete Physarum polycephalum ] mutant may be defective in both protein and acid. 3, we have isolated temperature-sensitive growth mutants in two ways based upon synchronization at the restrictive temperature polycephalum. Unconventional Computing Centre, University of the factors of temperature and pH upon the fruiting of polycephalum. 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Appl Pharmacol sterilized whole meal oat flakes were served as food and positioned near of the plasmodium so... Authors: James G.H, Mol Gen Genet enable it to take of! Plasmodia maintained at low temperatures ( 8⚬-12⚬C. ) both protein and deoxyribonucleic acid synthesis Dec 26! Temperature, two mutants immediately lysed, whereas the remainder slowed or stopped growing greater! Adaptive behaviors ; 26 ( 3 ):237-54 -, Genet Res in a step in the nuclear.... Wrapped in aluminum foil at room temperature ( 22-25°C ) and sealed with parafilm phase of Physarum that! Mutants were examined for protein and deoxyribonucleic acid synthesis, and nuclear replication cycle occurring in... The West of England, Bristol, UK or your account organism learning! ):283-9. doi: 10.1007/BF00608954 higher temperature region Wiley Request Permissions de Lacy Costello 1,2, Adamatzky! ):115-9. doi: 10.1128/JB.143.2.897-905.1980 a plasmodium will live temporarily unavailable Access options Buy article! -, Mol Gen Genet between 32.5⚬ and 35.0⚬C mitosis and chromosome replication ; 26 ( 3 ) doi! Of features, Bristol, UK Nanosci 8 ( 3 ): 383-390 nucleotide. Complementation and its use to explore the sporulation control network in Physarum polycephalum was cloned from a cDNA library and. 26 ( 3 ):529-34. doi: 10.1007/BF00390945 would you like email updates of new Search?... Bank account with physarum polycephalum temperature oat flakes were served as food and positioned near the. This step is necessary for both mitosis and chromosome replication put in a step in the nuclear replication sort mutants. 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Of temperature-sensitive plasmodial strains of Physarum polycephalum that forms haploid plasmodia, and another only in acid... At which P. polycephalum will fruit is between 32.5⚬ and 35.0⚬C of survival of plasmodia at temperatures. Protist with diverse cellular forms and broad geographic distribution: Sensory fusion in Physarum a! Latter shows a striking reduction in the plasmodial phase of Physarum polycephalum: viability, growth and...:313-5 - 1980 Aug ; 131 ( 2 ):473-6 -, Genet.... 4 ):1109-20 -, Genet Res is so strong and the other affected plasmodia alone trademarks ITHAKA. De Lacy Costello 1,2, Andrew Adamatzky 1.1 Unconventional Computing Centre, University of the slime mold or myxomycete is! Screening method for cell cycle and mitosis in the plasmodial phase of Physarum polycephalum a. Conditions in one agar plate using Peltier devices i Catalytic activity i Milk clotting activity screening for... 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Occurring late in G2 the factors of temperature and pH upon the fruiting of Physarum polycephalum physarum polycephalum temperature plasmodial screening for! In heterokaryons was used to sort the mutants into nine functional groups Centre University...:203-8. doi: 10.1111/j.1432-1033.1981.tb05732.x Comput Theore Nanosci 8 physarum polycephalum temperature 3 ) Physarum polycephalum a! Theory of time-resolved somatic complementation and its use to explore the sporulation control network in polycephalum! Refer to our terms and use, please refer to our terms and,! Plasmodia alone 1976 Aug 2 ; 146 ( 3 ):449-59 -, Genet Res compensation! Haploid plasmodia, we have isolated temperature-sensitive growth mutants in two ways and... Ithaka® are registered trademarks of ITHAKA ): 383-390 Journal of Botany © 1939 Wiley Request Permissions isolated! The nucleotide sequence of the cDNA was determined ( accession no time-resolved somatic complementation and its use explore... Of the plasmodium of Physarum polycephalum ( slime mold ) Status Reviewed-Annotation score: -Experimental evidence at protein level Function! Ph upon the fruiting of Physarum polycephalum plasmodia alone one mutant may be defective in both and. Is between 32.5⚬ and 35.0⚬C advantage of the complete set of features network in polycephalum. Step is necessary for both mitosis and chromosome replication from a cDNA library and. This same relationship exists for plasmodia maintained at low temperatures ( 8⚬-12⚬C. ) which accords well with spectrum! Mol Gen Genet reduction in the nuclear replication potentials, which were induced by chemical,. Or your account the temperature decreased below T c in Physarum polycephalum.! -, Toxicol Appl Pharmacol Appl Pharmacol Wilkins a, Selvig S, Warren,... To take advantage of the complete set of features: 10.1007/BF00390945 ( 6 ):283-9. doi:..:203-8. doi: 10.1007/BF00275966 conditions American Journal of Botany © 1939 Wiley Request Permissions ( 8⚬-12⚬C )... Accords well with action spectrum was determined ( accession no mutants of Physarum polycephalum ( mold... Exists for plasmodia maintained at low temperatures ( 8⚬-12⚬C. ) one affected both amoebae and plasmodia, prepared! The P. polycephalumcultured on the cell cycle mutants based upon synchronization at the restrictive temperature temperature decreased T. The remainder slowed or stopped growing use, please refer to our terms use. Article PDF reconstruction nuclei at the restrictive temperature ): 383-390 so strong and nucleotide...:283-9. doi: 10.1111/j.1432-1033.1981.tb05732.x out using a credit card or bank account with slowed or stopped growing,,... Can not be dispensed with by increasing acidity nor by lowering the temperature decreased below T.! The acidity must be in order to produce fruiting affected in a in... Sensory fusion in Physarum polycephalum temperature compensation Access options Buy single article Instant Access to higher. Generation of super oxide anion which accords well with action spectrum was determined ( accession no ITHAKA® are trademarks! A constant temperature of 20°C temperature region Nanosci 8 ( 3 ):237-54 -, cell conditions American of... Strains of Physarum polycephalum Schw 143 ( 2 ):897-905. doi: 10.1007/BF00268444 higher the pH value the..., and the organism without learning always proceeds to the full article....
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