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Use of microorganisms and fermenter to manufacture enzymes, Use of microorganisms to manufacture antibiotic penicillin, Main nutrients: carbohydrates, fats and proteins, Food test 2 - Benedict's test for Reducing Sugars, Food test 3 - Emulsion (ethanol) test for Fats, Other Nutrients: Vitamins, Minerals, Fiber and Water, Using microorganism in making yoghurt and single cell protein, Food additives - uses, benefits and health hazards, Use of modern technology for increasing food production, Problems of world food supplies and the causes of famine, Absorption – function of the small intestine and significance of villi, Photosynthesis investigations - Principles and Starch test, Effect of Light intensity on the rate of Photosynthesis, Effect of Temperature on the Rate of Photosynthesis, Effect of Carbon Dioxide on the Rate of Photosynthesis, Optimum conditions for photosynthesis in Green house, Plant's mineral requirements and fertilisers, Distribution of Xylem and Phloem in roots, stems and leaves, Passage of water through root, stem and leaf, Transpiration in plants and factors affecting tranpiration rate, Adaptations of the leaf, stem and root to different environments, Translocation of applied chemicals throughout the plant, Transport of materials from sources to sinks at different seasons, Effect of exercise on heartbeat and causes of a coronary heart disease, Arteries, veins and capillaries - structure and functions, Immune system - antibody, tissue rejection. 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Biotechnology and Genetic Engineering, Section 1: The Nature and Variety of Living Organisms, Section 2: Structures and Functions in Living Organisms, Module 1: Development of Practical Skills in Biology, Module 4: Biodiversity, Evolution and Disease, Module 5: Communication, Homeostasis and Energy, Module 6: Genetics, Evolution and Ecosystems, 3.3 Organisms exchange substances with their environment, 3.4 Genetic information, variation and relationships between organisms, 3.5 Energy transfers in and between organisms, 3.6 Organisms respond to changes in their internal and external environments, 3.7 Genetics, populations, evolution and ecosystems, Topic 4: Biodiversity and Natural Resources, Topic 6: Immunity, Infection and Forensics, Topic 8: Metabolism, Cell Respiration and Photosynthesis, 1.2) Concept and use of classification system, Anaerobic respiration – Ethanol and Lactate pathways, ATP yield in aerobic and anaerobic respiration, 16.7) Sexually transmitted infections (STIs), 20.1) Biotechnology and genetic engineering, B1.6 Waste materials from plants and animals. 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